pet28 dnmt3a3l sc27 ar tic le (Addgene inc)
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pet28 dnmt3a3l sc27 ar tic le
Pet28 Dnmt3a3l Sc27 Ar Tic Le, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28+dnmt3a3l+sc27/pET28-Dnmt3a3L-sc27+(Plasmid+%2371827)/pm41620608-257-20-29
Average 93 stars, based on 11 article reviews
Pet28 Dnmt3a3l Sc27 Ar Tic Le, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet28+dnmt3a3l+sc27/pET28-Dnmt3a3L-sc27+(Plasmid+%2371827)/pm41620608-257-20-29
Average 93 stars, based on 11 article reviews
pet28 dnmt3a3l sc27 ar tic le - by Bioz Stars,
2026-10
93/100 stars
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Amplification:Article Title: Systematic Epigenome Editing Captures the Context-dependent Instructive Function of Chromatin Modifications Article Snippet: For all chromatin-modifying ‘effector’ plasmids, the scFV domain and a superfolder (sf)GFP coding sequence were amplified from the PlatTET-gRNA2 plasmid (Addgene #82559) and fused in frame with the catalytic domain (CD) or the full-length (FL) of mouse Prdm9, P300, Dot1L, G9a, Kmt5c, Setd2, Ezh2 and Ring1b , all amplified from early passage ESC cDNA. .. Dnmt3a CD and the C-terminal part of mouse Dnmt3L (3a3L) were amplified from Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications Article Snippet: For all chromatin-modifying ‘effector’ plasmids, the sequence for the scFV domain and an sfGFP coding sequence were amplified from the PlatTET-gRNA2 plasmid (Addgene, 82559) and fused in frame with the CD or FL mouse Prdm9 , p300 , Dot1l , G9a , Kmt5c , Setd2 , Ezh2 and Ring1b , all amplified from early-passage ESC cDNA. .. Sequences for the Dnmt3a CD and the C-terminal part of mouse Dnmt3a (3a3l) were amplified from Article Title: Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications. Article Snippet: Sequences for the Dnmt3a CD and the C-terminal part of mouse Dnmt3a (3a3l) were amplified from pET28-Dnmt3a3l-sc27 (Addgene, 71827). .. Sequences for the Dnmt3a CD and the C-terminal part of mouse Dnmt3a (3a3l) were amplified from Article Title: Designer Receptors for Nucleotide‐Resolution Analysis of Genomic 5‐Methylcytosine by Cellular Imaging Article Snippet: PCR fragment and vector pAnI5211 were digested with NdeI and BsrGI enzymes and then ligated with T4 ligase (New England Biolabs, M0202T) using 3:1 insert:vector molar ratio. pJaW876 was cloned by Gibson assembly2. .. For this, vector pcDNA3.1-GoldenGate-VP64 (Addgene, #47389) was linearized by PCR using primers o2079 and o2080 and the insert DNMT3a3L CD was amplified by PCR using Article Title: Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Article Snippet: For generation of plasmids with scFv-linked EDs encoding the catalytic domain (CD) of DNMT3A, DNMT3A3L, KRAB and LSD1, the TET1 plasmid was digested for 1 h at 37 ◦C with FD-NotI and with FD-NheI (both Thermo Fisher Scientific). .. The sequences of DNMT3A, DNMT3A3L, KRAB and LSD1 were PCR amplified from pdCas9DNMT3A-EGFP (Addgene #71666), Polymerase Chain Reaction:Article Title: Designer Receptors for Nucleotide‐Resolution Analysis of Genomic 5‐Methylcytosine by Cellular Imaging Article Snippet: PCR fragment and vector pAnI5211 were digested with NdeI and BsrGI enzymes and then ligated with T4 ligase (New England Biolabs, M0202T) using 3:1 insert:vector molar ratio. pJaW876 was cloned by Gibson assembly2. .. For this, vector pcDNA3.1-GoldenGate-VP64 (Addgene, #47389) was linearized by PCR using primers o2079 and o2080 and the insert DNMT3a3L CD was amplified by PCR using Article Title: Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Article Snippet: For generation of plasmids with scFv-linked EDs encoding the catalytic domain (CD) of DNMT3A, DNMT3A3L, KRAB and LSD1, the TET1 plasmid was digested for 1 h at 37 ◦C with FD-NotI and with FD-NheI (both Thermo Fisher Scientific). .. The sequences of DNMT3A, DNMT3A3L, KRAB and LSD1 were PCR amplified from pdCas9DNMT3A-EGFP (Addgene #71666), Plasmid Preparation:Article Title: Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Article Snippet: .. pSPgRNA was a gift from Charles Gersbach, pCAG-dCas95xPlat2AflD and pCAG-scFvGCN4sfGFPTET1CD were gifts from Izuho Hatada, pdCas9-DNMT3A-EGFP was a gift from Vlatka Zoldoš, pSpCas9 (BB)-2A-GFP (PX458) was a gift from Feng Zhang (Addgene plasmid # 48138), |